{
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"FrameKey-0-0-0": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "d8fd658d-22ff-4294-80ea-0aa3dffa2028",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 0,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 0,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 184563,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_000.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 85,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:54 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-1": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "c09ce985-be9c-473b-8975-870cbf7936fd",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 1,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 1,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 184815,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_001.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 90,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:54 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-2": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "e441cf66-02b9-4b1a-a369-2ae1b846399b",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 2,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 2,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 185067,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_002.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 95,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:54 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-3": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "de9c27eb-2867-40e8-a9cc-e07721d0dd64",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 3,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 3,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 185318,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_003.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 100,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:55 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-4": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "4741e113-784f-4d11-8118-8149c2d2261c",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 4,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 4,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 185571,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_004.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 105,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:55 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-5": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 0,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "be00bc2b-1a4a-4bf6-8a84-2c8e5cc13ae4",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 5,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 5,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 185823,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_005.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 110,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:55 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-6": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "a7fcb272-df8f-47b2-8d5f-ac5bba2859dc",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 6,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 6,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 186076,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_006.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 115,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:55 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-7": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "8e027518-49d9-48f1-af1e-e8ca7fc5e441",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 7,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 7,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 186329,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_007.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 120,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:56 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-8": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "4d3567b2-19f4-4e9f-9d5f-727879c17742",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 8,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 8,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 186580,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_008.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 125,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:56 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-9": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "118016d4-c95d-4d73-b276-915b91e2dba8",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 9,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 9,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 186832,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_009.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 130,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:56 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-10": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "7719420e-2c84-4758-ab16-67e5b00bf961",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 10,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 10,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 187084,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_010.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 135,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:56 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-0": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "213cef54-716c-4cd0-a4a4-3966d6cb1c16",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 0,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 0,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 188001,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_000.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 85,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:57 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-1": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "60eef6da-e2a4-401e-82ee-cd7a98c08ba9",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 1,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 1,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 188173,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_001.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 90,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:57 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-2": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "1e2da62d-fbdc-4728-8576-64cf3fe4a4e8",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 2,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 2,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 188344,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_002.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 95,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:58 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-3": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "da7f5b16-f5cb-4cc1-9875-b456032f9a31",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 3,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 3,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 188516,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_003.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 100,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:58 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-4": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "a862e144-edc2-4fb6-8b00-d60a65a04657",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 4,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 4,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 188704,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_004.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 105,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:58 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-5": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 0,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "b02cdd32-5534-45f7-9fb9-2df899d00c14",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 5,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 5,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 188876,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_005.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 110,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:58 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-6": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "c393d008-acfe-481d-88af-61e349fccd5e",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 6,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 6,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 189047,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_006.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 115,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:58 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-7": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "7ff6b8d1-5ee2-4a73-892c-3f8b7873ff02",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 7,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 7,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 189218,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_007.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 120,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:59 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-8": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "cee83709-af44-4364-ac3f-767caff1d0d5",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 8,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 8,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 189390,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_008.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 125,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:59 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-9": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "376e84eb-ae73-46a5-9cb9-a9b165cbabdb",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 9,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 9,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 189562,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_009.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 130,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:59 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-10": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "a9f928c1-bbce-4a25-96ab-a41ca75d6877",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 10,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 10,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 189733,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_010.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 135,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:42:59 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-0": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "b8f365d0-38f9-4738-be3c-c939f940888d",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 0,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 0,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 190679,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_000.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 85,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:00 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-1": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "e96ae812-fafa-44dd-ac31-f517f6536f38",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 1,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 1,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 190930,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_001.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 90,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:00 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-2": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "7e0a12fd-91bb-4ef3-8009-34bccebfef92",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 2,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 2,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 191183,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_002.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 95,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:00 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-3": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "ee0a46f3-c414-490f-8abf-99e6e8e412ca",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 3,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 3,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 191434,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_003.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 100,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:01 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-4": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "6d8c06d3-3fc3-4e3d-9e6f-3ad462c37d93",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 4,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 4,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 191686,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_004.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 105,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:01 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-5": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 0,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "7ce9001a-8dd9-4ee9-8d87-a802cbaeff3b",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 5,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 5,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 191938,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_005.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 110,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:01 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-6": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "fd10d711-57fc-4b92-91a3-ca999eb6fdc1",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 6,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 6,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 192199,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_006.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 115,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:01 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-7": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "a0be0c3c-ee2f-4f3f-b204-77dfe2072916",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 7,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 7,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 192451,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_007.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 120,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:02 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-8": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "c5452f7a-4ee6-4357-b30a-3f1609b3d3a5",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 8,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 8,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 192702,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_008.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 125,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:02 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-9": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "09f876de-d7f5-4704-bb7d-1f4830f42495",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 9,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 9,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 192952,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_009.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 130,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:02 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-10": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "10dcb9b9-c3d8-4e68-a35e-44c6992c4586",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 10,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 10,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 193203,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_010.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 135,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:02 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-0": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "ddb08179-c805-48c4-9755-35cabb88a939",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 0,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 0,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 193832,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_000.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 85,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:03 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-1": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "60463672-310e-4ba8-bfa9-b9ddb6f9a3e3",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 1,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 1,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 194184,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_001.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 90,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:03 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-2": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "3ca72c8d-c4ba-4e1d-8c2d-5984e94b7887",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 2,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 2,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 194536,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_002.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 95,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:04 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-3": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "8d0d52e4-9ea0-4f70-97bc-e39656fc60a3",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 3,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 3,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 194888,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_003.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 100,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:04 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-4": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "7ba8513a-a057-433b-b82f-6b35121c8506",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 4,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 4,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 195240,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_004.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 105,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:05 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-5": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 0,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "fa3c09fe-bfce-491a-badb-7b06521943c1",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 5,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 5,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 195591,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_005.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 110,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:05 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-6": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "cde65409-2bbd-450d-b0e8-643228768a53",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 6,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 6,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 195942,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_006.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 115,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:05 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-7": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "67d1d2e2-8129-4b2b-abe6-dd028713480f",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 7,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 7,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 196293,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_007.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 120,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:06 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-8": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "0b376249-7e46-48d8-ade3-e87d89e68f8b",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 8,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 8,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 196644,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_008.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 125,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:06 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-9": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "87695b0c-1e36-4b96-88b8-78356a5b3121",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 9,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 9,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 197009,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_009.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 130,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:06 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-10": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "5d35d7d6-e2b1-4547-8c83-71dd7148e554",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 10,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 10,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 197360,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_010.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 135,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:07 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-0": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "0a215b7d-fb39-412c-a6a8-c8762bacccd2",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 0,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 0,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 198464,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_000.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 85,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:08 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-1": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "bb84cdda-ff9b-4c41-a0ca-84d49dbd0c3a",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 1,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 1,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 198897,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_001.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 90,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:08 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-2": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "2224fc17-9f62-46ed-8663-52431607cf3c",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 2,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 2,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 199329,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_002.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 95,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:09 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-3": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "27670b2f-8e85-4c16-b667-673f0c78883e",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 3,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 3,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 199761,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_003.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 100,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:09 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-4": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "c6541ab6-48f5-41ab-8884-bc95b8e80cf8",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 4,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 4,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 200193,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_004.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 105,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:09 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-5": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 0,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "15b61744-b56d-453a-9086-6cee25f5ab41",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 5,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 5,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 200640,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_005.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 110,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:10 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-6": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "b9880d6e-b984-48e9-a76b-bc29647e5701",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 6,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 6,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 201088,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_006.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 115,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:10 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-7": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "53651b9d-b61f-4bb1-85f0-81c3e515855f",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 7,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 7,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 201503,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_007.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 120,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:11 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-8": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "4e288835-e98a-4aae-a198-5c7c2e047ffd",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 8,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 8,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 201936,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_008.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 125,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:11 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-9": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "3155ac7e-fd0c-40a1-9dc5-bb0a9a7acc09",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 9,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 9,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 202351,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_009.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 130,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:12 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-10": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.8100",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "1794bdfd-e823-45a7-9af5-c4b515754d2f",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 9,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 11,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8626.03],
"DA Z Stage": [110],
"XYStage": [
56674,
-4686
]
},
"GridColumnIndex": 0,
"Label": "Pos0_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.75],
"DA Z Stage": [110],
"XYStage": [
57372,
1276
]
},
"GridColumnIndex": 0,
"Label": "Pos1_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.6],
"DA Z Stage": [110],
"XYStage": [
57094,
19672
]
},
"GridColumnIndex": 0,
"Label": "Pos2_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.9],
"DA Z Stage": [110],
"XYStage": [
57424,
26736
]
},
"GridColumnIndex": 0,
"Label": "Pos3_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.73],
"DA Z Stage": [110],
"XYStage": [
65429,
28068
]
},
"GridColumnIndex": 0,
"Label": "Pos4_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8578.3],
"DA Z Stage": [110],
"XYStage": [
65567,
21813
]
},
"GridColumnIndex": 2,
"Label": "Pos5_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8608.36],
"DA Z Stage": [110],
"XYStage": [
65430,
3522
]
},
"GridColumnIndex": 2,
"Label": "Pos6_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8619.85],
"DA Z Stage": [110],
"XYStage": [
64769,
-1886
]
},
"GridColumnIndex": 2,
"Label": "Pos7_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8584.95],
"DA Z Stage": [110],
"XYStage": [
109,
-5416
]
},
"GridColumnIndex": 0,
"Label": "Pos8_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.68],
"DA Z Stage": [110],
"XYStage": [
236,
438
]
},
"GridColumnIndex": 2,
"Label": "Pos9_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8564.23],
"DA Z Stage": [110],
"XYStage": [
534,
19929
]
},
"GridColumnIndex": 2,
"Label": "Pos10_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8563.93],
"DA Z Stage": [110],
"XYStage": [
968,
25006
]
},
"GridColumnIndex": 2,
"Label": "Pos11_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8554.79],
"DA Z Stage": [110],
"XYStage": [
8063,
28294
]
},
"GridColumnIndex": 2,
"Label": "Pos12_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.53],
"DA Z Stage": [110],
"XYStage": [
8981,
22504
]
},
"GridColumnIndex": 2,
"Label": "Pos13_002_002",
"GridRowIndex": 2
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.92],
"DA Z Stage": [110],
"XYStage": [
8176,
4489
]
},
"GridColumnIndex": 0,
"Label": "Pos14_002_002",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8582.44],
"DA Z Stage": [110],
"XYStage": [
9096,
-1640
]
},
"GridColumnIndex": 2,
"Label": "Pos15_002_002",
"GridRowIndex": 2
}
],
"Interval_ms": 0,
"UUID": "00708ba0-1c4c-4c4e-b124-cfa2fa3ffcd5",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-20 00:42:54 -0500",
"Date": "2019-12-20",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 9,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq10x_6_2",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": false,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 16,
"Directory": "F:\\20191216M1cdhs"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 10,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "10001000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 10,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "0.0000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "0.0000",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 202783,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos9_002_002",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "12937600.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "7",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 236,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_010.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 135,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "8",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "2",
"NextFrame": 0,
"ManualFocus-Position": "8582.4400",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-20 00:43:12 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "0",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 438,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "3.0000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
}
}