{
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"FrameKey-0-0-0": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -40,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "1",
"UUID": "d39b4779-2591-4444-ad64-e128a137ed5b",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 0,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 0,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 340311,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_000.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 70,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:19 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-1": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -35,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "1",
"UUID": "2a8e56f8-48df-4b5b-84ac-7b2c3651cdfe",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 1,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 1,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 340552,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_001.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 75,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:19 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-2": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -30,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "1",
"UUID": "c5bd09f9-826f-45b6-892c-c79b91f1b09a",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 2,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 2,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 340792,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_002.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 80,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:19 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-3": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "1",
"UUID": "27be4b06-3b31-4c9f-921e-0a13460058d7",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 3,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 3,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 341034,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_003.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 85,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:19 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-4": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "1",
"UUID": "8a946cc2-3d6a-435c-bd6b-fd64bca503e1",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 4,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 4,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 341276,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_004.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 90,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:20 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-5": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "1",
"UUID": "20a7ac6a-ea41-4480-83ef-ccb442bcc065",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 5,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 5,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 341516,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_005.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 95,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:20 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-6": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "1",
"UUID": "0347f459-dea1-4d9f-a5f7-2008a05fc697",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 6,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 6,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 341757,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_006.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 100,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:20 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-7": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "1",
"UUID": "86b9dd68-9c8a-47ee-b8fc-fce6a6b90a6a",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 7,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 7,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 341997,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_007.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 105,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:20 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-8": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 0,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "1",
"UUID": "e3134c86-ea5d-4704-9aa0-a05c1cff91bb",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 8,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 8,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 342239,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_008.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 110,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:21 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-9": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "1",
"UUID": "afebf46d-de69-43b7-a22c-0957babeb066",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 9,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 9,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 342479,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_009.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 115,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:21 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-10": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "1",
"UUID": "65c2d7a8-2c45-4758-beff-c35ff24b9f5b",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 10,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 10,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 342723,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_010.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 120,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:21 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-11": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "1",
"UUID": "505ef9c4-ba37-4a39-8a28-782e9031dc18",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 11,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 11,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 342967,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_011.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 125,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:21 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-12": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "1",
"UUID": "da552b4c-4f08-41c8-b3ac-7299362553c1",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 12,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 12,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 343209,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_012.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 130,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:22 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-13": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "1",
"UUID": "8fdc2176-736c-4b59-9216-88ed3737c4db",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 13,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 13,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 343449,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_013.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 135,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:22 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-14": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 30,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "1",
"UUID": "6cd5f46c-0f45-4395-9db3-13b27c62434a",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 14,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 14,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 343690,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_014.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 140,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:22 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-15": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 35,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "1",
"UUID": "590e7201-f1a6-4185-9f80-e9176fbe16d8",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 15,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 15,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 343931,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_015.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 145,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:22 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-0-16": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "5.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 40,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "0a385ea0-48a9-4cdd-89d8-e0356a2ce4a0",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "CFP/YFP",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "565/24",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "5.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 16,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 16,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "520",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 344176,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-G_016.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 150,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-G",
"XLIGHT Excitation Wheel-Label": "CFP/YFP",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:23 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 0,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-0": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -40,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "d85bd285-6a22-451a-986f-8cd358a03789",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 0,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 0,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 345093,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "1",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_000.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 70,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:24 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-1": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -35,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "e3305229-101b-41d4-a7bb-d53a3eb92483",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 1,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 1,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 345253,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "1",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_001.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 75,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:24 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-2": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -30,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "bf024aa4-67eb-4b3d-939d-7196d1d2c289",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 2,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 2,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 345413,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "1",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_002.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 80,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:24 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-3": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "7a4f163f-faf9-4683-bb10-45f8417793a9",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 3,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 3,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 345578,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "1",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_003.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 85,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:24 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-4": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "dcb0500d-828a-4733-a98f-4b310c039256",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 4,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 4,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 345738,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "1",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_004.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 90,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:24 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-5": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "e39dc003-9bcb-4bf7-bcc3-1bdfb2a1773a",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 5,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 5,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 345899,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "1",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_005.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 95,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:24 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-6": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "3af780a8-a1b0-462e-ab74-005a7cea8e95",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 6,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 6,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 346059,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "1",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_006.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 100,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:25 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-7": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "a95f071f-ffe9-451c-9197-ec72f9991595",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 7,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 7,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 346221,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "1",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_007.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 105,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:25 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-8": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 0,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "6ef62c3f-4918-4f52-8fe0-18d08d60024e",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 8,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 8,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 346381,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "1",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_008.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 110,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:25 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-9": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "8b930cd9-e8ff-4809-8f05-17e3a1c652cf",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 9,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 9,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 346544,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "1",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_009.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 115,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:25 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-10": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "42761bf4-d378-4342-8bc3-13c31651e5d0",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 10,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 10,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 346704,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "1",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_010.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 120,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:25 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-11": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "1808b75a-4102-41eb-9589-8fa120369305",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 11,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 11,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 346866,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "1",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_011.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 125,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:25 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-12": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "b2cfc958-92f6-4200-8746-61fc59759b46",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 12,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 12,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 347027,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "1",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_012.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 130,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:25 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-13": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "488d058f-8086-4666-bb4c-18156fbd54a0",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 13,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 13,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 347188,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "1",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_013.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 135,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:26 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-14": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 30,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "e871acd2-934a-4efb-a1a8-99f976bad7c7",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 14,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 14,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 347349,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "1",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_014.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 140,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:26 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-15": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 35,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "9fcd36fe-faed-40fa-baa8-fe347d6ea588",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 15,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 15,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 347510,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "1",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_015.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 145,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:26 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-1-16": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 40,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 80,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "b0cb481b-b0ae-4398-a2ee-39354b68fbd8",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "585/11",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 16,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 16,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "555",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 347675,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "80.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-T_016.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 150,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-T",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:26 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 1,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "5.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "5.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-0": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -40,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "188070b3-d495-4b4c-a627-fbefaa048538",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 0,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 0,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 348628,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_000.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 70,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:27 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-1": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -35,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "2b83e67e-ae10-4bbd-a7c0-0134684da3b7",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 1,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 1,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 348869,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_001.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 75,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:27 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-2": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -30,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "5e5686c6-f379-4c14-9d5e-44f9e1cf7c55",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 2,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 2,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 349110,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_002.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 80,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:28 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-3": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "af1654c8-d9fa-428f-b472-41e6bacee8fa",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 3,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 3,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 349352,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_003.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 85,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:28 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-4": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "f38a3bd9-6320-4e24-b413-99b279cd7b2c",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 4,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 4,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 349593,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_004.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 90,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:28 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-5": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "cf8ba830-6780-4904-ac21-8b12dc53a8b3",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 5,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 5,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 349834,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_005.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 95,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:28 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-6": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "98431e15-7f6f-469a-a845-69ad7ab11c92",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 6,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 6,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 350075,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_006.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 100,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:29 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-7": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "d3f9c933-5454-453e-885f-b9e7fac50e7b",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 7,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 7,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 350316,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_007.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 105,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:29 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-8": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 0,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "a19d17c6-9078-4d16-af31-aaed8737af78",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 8,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 8,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 350558,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_008.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 110,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:29 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-9": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "6a525529-4968-4ad9-8905-16fe628a482b",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 9,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 9,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 350807,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_009.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 115,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:29 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-10": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "029c416b-8afa-4d42-8219-d0273003442e",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 10,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 10,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 351048,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_010.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 120,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:29 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-11": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "66270cbf-af83-48e7-bd08-d96ec4ca2bdb",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 11,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 11,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 351289,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_011.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 125,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:30 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-12": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "6da1ca8a-d264-4270-a0fc-9100b120b619",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 12,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 12,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 351529,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_012.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 130,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:30 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-13": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "be959b6e-65e9-4358-b366-5fbc847d556a",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 13,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 13,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 351770,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_013.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 135,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:30 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-14": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 30,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "cba6def9-5e5a-4fc6-9036-b600e8e6219e",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 14,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 14,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 352012,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_014.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 140,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:30 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-15": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 35,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "ba324b43-9cc5-4e58-988a-a72158dee7bb",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 15,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 15,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 352254,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_015.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 145,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:31 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-2-16": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 40,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 160,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "cea21594-b320-4d9d-8f1c-bf3783231b71",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "676/29",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 16,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 16,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 352500,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "160.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-A_016.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 150,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-A",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:31 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 2,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-0": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -40,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "16341d02-8084-4e35-b94f-d256a37cd20a",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 0,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 0,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 353125,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_000.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 70,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:32 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-1": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -35,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "41d07bec-37b2-4d24-b586-02a4065b42c2",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 1,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 1,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 353466,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_001.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 75,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:32 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-2": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -30,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "d5c024b5-9c52-4ea7-984d-724eb7d60471",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 2,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 2,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 353815,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_002.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 80,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:32 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-3": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "6ce731fd-1fba-43d7-8c6c-9a8d76f9ca5b",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 3,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 3,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 354156,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_003.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 85,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:33 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-4": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "7026b021-134d-4e70-9460-6b747607f347",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 4,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 4,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 354497,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_004.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 90,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:33 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-5": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "2c52bab2-db96-4e31-a919-5213c74c86e5",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 5,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 5,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 354838,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_005.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 95,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:33 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-6": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "45f4d664-5a90-4dbc-99c6-869f518fc7df",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 6,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 6,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 355179,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_006.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 100,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:34 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-7": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "7c02ebe6-0b2e-4d24-8d67-d247a9f8fc31",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 7,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 7,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 355519,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_007.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 105,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:34 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-8": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 0,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "5fa0936d-66d0-4d18-a8ee-ba019a76f4e9",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 8,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 8,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 355864,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_008.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 110,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:34 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-9": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "a862eebb-08bc-4cbc-ae9c-bb701f7254bd",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 9,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 9,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 356205,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_009.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 115,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:35 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-10": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "a0ea6df8-26e6-43eb-be0e-e58fd69a9cb2",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 10,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 10,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 356545,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_010.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 120,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:35 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-11": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "91be17d2-12ed-40bb-b47c-f0fe1960b48a",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 11,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 11,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 356893,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_011.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 125,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:35 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-12": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "7b213ff1-d2ac-44c3-96da-f3e1a9321ba1",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 12,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 12,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 357234,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_012.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 130,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:36 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-13": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "28e51c1a-b152-4e3b-8f33-2e4bc6107427",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 13,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 13,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 357576,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_013.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 135,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:36 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-14": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 30,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "7abf5264-b140-4812-8bd6-6a3d08b88f90",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 14,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 14,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 357917,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_014.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 140,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:36 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-15": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 35,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "beccf1b8-16b6-4ef9-ad0a-9bb40a85f755",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 15,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 15,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 358257,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_015.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 145,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:37 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "1",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-3-16": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 40,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 260,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "cce4ef86-685d-4c19-93c7-a06b39787b68",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "FF652-Di01",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "725/40",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 16,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 16,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "640",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 358605,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "260.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "5.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_Il-C_016.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 150,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "Il-C",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:37 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 3,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "5.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-0": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -40,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "c44b4bb7-4cd9-47ce-9651-22489ebed332",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 0,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 0,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 359564,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_000.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 70,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:38 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "1",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-1": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -35,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "fc26e230-5b91-49de-90a2-2e4d7595b494",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 1,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 1,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 359724,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_001.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 75,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:38 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "1",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-2": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -30,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "46618643-cd84-4372-b6ce-bfa0775faffc",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 2,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 2,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 359883,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_002.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 80,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:38 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "1",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-3": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "c5cc0bbb-15a2-4111-98b1-e7fad59fd4bd",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 3,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 3,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 360045,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_003.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 85,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:38 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "1",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-4": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "263fb576-de2f-4cc9-ad49-5f35dbd75171",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 4,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 4,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 360219,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_004.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 90,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:39 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "1",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-5": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "c1e0cebf-dcb2-4e44-a1b7-4ebff8923919",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 5,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 5,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 360380,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_005.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 95,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:39 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "1",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-6": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "7a768317-c615-424c-bfdd-399679669e77",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 6,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 6,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 360539,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_006.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 100,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:39 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "1",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-7": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": -5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "9b3aa456-a697-4de0-b3c2-ec66878579ac",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 7,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 7,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 360699,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_007.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 105,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:39 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "1",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-8": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 0,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "2f7839df-57c1-4669-9817-6394f0565c13",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 8,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 8,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 360861,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_008.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 110,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:39 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "1",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-9": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 5,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "16c5930b-09c7-41f0-8a41-1488c2a28ce2",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 9,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 9,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 361020,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_009.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 115,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:39 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "1",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-10": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 10,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "a8adb143-d16b-4cc5-9e5d-9ffecae2b1ae",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 10,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 10,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 361179,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_010.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 120,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:40 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "1",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-11": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 15,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "ef832e62-98ea-4ce0-9b59-bd493485e28a",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 11,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 11,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 361341,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_011.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 125,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:40 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "1",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-12": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 20,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "f13cf892-8891-4d3e-abb0-9b29a91ac6ff",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 12,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 12,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 361499,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_012.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 130,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:40 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "1",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-13": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 25,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "93d6f2f5-feec-48b1-b5ed-8a7c490cc624",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 13,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 13,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 361660,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_013.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 135,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:40 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "1",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-14": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 30,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "a18f877f-4e62-4b08-85b1-25ecc5fc8b3f",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 14,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 14,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 361821,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_014.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 140,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:40 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "1",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-15": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 35,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "d84d24aa-d750-41d1-8bc8-bfb796fe2a81",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 15,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 15,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 361979,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_015.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 145,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:40 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "1",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
},
"FrameKey-0-4-16": {
"Objective-Name": "Objective",
"Condenser-Name": "Condenser",
"Core-Initialize": "1",
"405-DA Device": "405 Shutter",
"470 Shutter-Block voltage": "false",
"555-Name": "DA Shutter",
"640 AO-Demo": "No",
"405 Shutter-MaxVolts": "5.0000",
"ManualFocus-Name": "ManualFocus",
"470-Name": "DA Shutter",
"520 AO-Volts": "0.0000",
"ROI": "0-0-2048-2048",
"520 AO-Description": "NI DAC",
"Prime BSI-PP 2 THRESHOLD": "120",
"Prime BSI-Port": "CMOS",
"555 Shutter-MaxVolts": "5.0000",
"470 AO-Description": "NI DAC",
"470-State": "0",
"LudlShutter-Description": "Ludl shutter adapter",
"Prime BSI-PP 5 NO OF ITERATIONS": "2",
"SlicePosition": 40,
"Prime BSI-CCDTemperatureSetPoint": "-20.0000",
"640 Shutter-TriggerSampleRate": "100000",
"Exposure-ms": 10,
"405 AO-MaxVolts": "5.0000",
"COM6-DelayBetweenCharsMs": "0.0000",
"Prime BSI-PP 4 DENOISING": "DENOISING",
"640-Description": "DA device that is used as a shutter",
"445 Shutter-Volts": "0.0000",
"XLIGHT Disk slider-Description": "XLIGHT Disk slider",
"640 AO-Sequenceable": "1",
"405-Description": "DA device that is used as a shutter",
"445-DA Device": "445 Shutter",
"PI AO-Demo": "No",
"470 AO-MinVolts": "0.0000",
"LudlShutter-LudlShutterNumber": "1",
"555 Shutter-MinVolts": "0.0000",
"Prime BSI-FanSpeedSetpoint": "High",
"405 AO-MinVolts": "0.0000",
"AxisPositions": null,
"DA Z Stage-Stage Low Voltage": "0.0000",
"Prime BSI-Name": "PMPCIECam00",
"Prime BSI-FTCapable": "No",
"XLIGHT Spin motor-Description": "XLIGHT Disk spin motor",
"520 Shutter-AutoDetectedOutputPort": "Dev1/ao10",
"520 AO-TriggerInputLine": "/Dev1/PFI0",
"PI AO-TriggerSequenceLength": "1000",
"555 Shutter-AutoDetectedOutputPort": "Dev1/ao12",
"XYStage-Port": "COM4",
"XYStage-MaxSpeed": "100",
"445 Shutter-IOChannel": "Dev1/ao8",
"Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW",
"Prime BSI-CCDTemperature": "-19.7600",
"Prime BSI-SMARTStreamingEnabled": "No",
"Objective-ClosedPosition": "",
"640 Shutter-IOChannel": "Dev1/ao13",
"520 AO-TriggerSequenceLength": "1000",
"528 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-ShutterMode": "Pre-Exposure",
"445-Description": "DA device that is used as a shutter",
"445-Name": "DA Shutter",
"COM3-Handshaking": "Off",
"405 AO-Description": "NI DAC",
"520-State": "0",
"UUID": "688a5891-a542-4f4c-9418-a707ccbf89dd",
"Core-Camera": "Prime BSI",
"Core-SLM": "",
"528 AO-Block voltage": "false",
"470 Shutter-MinVolts": "0.0000",
"XLIGHT Disk slider-State": "1",
"Core-ChannelGroup": "Channels1",
"PositionIndex": 14,
"WaitInterval": null,
"XLIGHT Disk slider-Label": "Disk Pos50um",
"DA Z Stage-Name": "DA Z Stage",
"555-DA Device": "555 Shutter",
"Prime BSI-X-dimension": "2048",
"470 AO-MaxVolts": "5.0000",
"445 Shutter-Demo": "No",
"Prime BSI-CircularBufferFrameRecovery": "OFF",
"445 AO-TriggerSampleRate": "100000",
"Prime BSI-PVCAM Adapter Version": "1.3.39",
"528 AO-Description": "NI DAC",
"470 Shutter-SupportsTriggering": "0",
"528 AO-MinVolts": "0.0000",
"528 Shutter-Sequenceable": "1",
"470 AO-TriggerSequenceLength": "1000",
"520 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-PP 4 THRESHOLD": "85",
"Summary": {
"Slices": 17,
"InitialPositionList": [
{
"DeviceCoordinatesUm": {
"ManualFocus": [8598.36],
"DA Z Stage": [110],
"XYStage": [
56036,
-3134
]
},
"GridColumnIndex": 0,
"Label": "Pos0",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8588],
"DA Z Stage": [110],
"XYStage": [
66300,
-3308
]
},
"GridColumnIndex": 0,
"Label": "Pos1",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8583.58],
"DA Z Stage": [110],
"XYStage": [
56141,
2334
]
},
"GridColumnIndex": 0,
"Label": "Pos2",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8573.45],
"DA Z Stage": [110],
"XYStage": [
66418,
2426
]
},
"GridColumnIndex": 0,
"Label": "Pos3",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8558.68],
"DA Z Stage": [110],
"XYStage": [
55516,
22579
]
},
"GridColumnIndex": 0,
"Label": "Pos4",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8567.76],
"DA Z Stage": [110],
"XYStage": [
65690,
22248
]
},
"GridColumnIndex": 0,
"Label": "Pos5",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8565.17],
"DA Z Stage": [110],
"XYStage": [
57290,
28805
]
},
"GridColumnIndex": 0,
"Label": "Pos6",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8570.81],
"DA Z Stage": [110],
"XYStage": [
67143,
28051
]
},
"GridColumnIndex": 0,
"Label": "Pos7",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8574.19],
"DA Z Stage": [110],
"XYStage": [
-1233,
-3154
]
},
"GridColumnIndex": 0,
"Label": "Pos8",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8575.8],
"DA Z Stage": [110],
"XYStage": [
9073,
-3048
]
},
"GridColumnIndex": 0,
"Label": "Pos9",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8576.98],
"DA Z Stage": [110],
"XYStage": [
-634,
3072
]
},
"GridColumnIndex": 0,
"Label": "Pos10",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8587.42],
"DA Z Stage": [110],
"XYStage": [
9525,
2684
]
},
"GridColumnIndex": 0,
"Label": "Pos11",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8552.77],
"DA Z Stage": [110],
"XYStage": [
-401,
22627
]
},
"GridColumnIndex": 0,
"Label": "Pos12",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8524.74],
"DA Z Stage": [110],
"XYStage": [
9661,
22822
]
},
"GridColumnIndex": 0,
"Label": "Pos13",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8559.38],
"DA Z Stage": [110],
"XYStage": [
414,
28632
]
},
"GridColumnIndex": 0,
"Label": "Pos14",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8546.17],
"DA Z Stage": [110],
"XYStage": [
9552,
28686
]
},
"GridColumnIndex": 0,
"Label": "Pos15",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8507.19],
"DA Z Stage": [110],
"XYStage": [
-17798,
40802
]
},
"GridColumnIndex": 0,
"Label": "Pos16",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8490.75],
"DA Z Stage": [110],
"XYStage": [
-28175,
40389
]
},
"GridColumnIndex": 0,
"Label": "Pos17",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8512.15],
"DA Z Stage": [110],
"XYStage": [
-17650,
34874
]
},
"GridColumnIndex": 0,
"Label": "Pos18",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8487.65],
"DA Z Stage": [110],
"XYStage": [
-28213,
34554
]
},
"GridColumnIndex": 0,
"Label": "Pos19",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8518.5],
"DA Z Stage": [110],
"XYStage": [
-18036,
15301
]
},
"GridColumnIndex": 0,
"Label": "Pos20",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8497.89],
"DA Z Stage": [110],
"XYStage": [
-28392,
15409
]
},
"GridColumnIndex": 0,
"Label": "Pos21",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8527.26],
"DA Z Stage": [110],
"XYStage": [
-18249,
9123
]
},
"GridColumnIndex": 0,
"Label": "Pos22",
"GridRowIndex": 0
},
{
"DeviceCoordinatesUm": {
"ManualFocus": [8504.32],
"DA Z Stage": [110],
"XYStage": [
-28811,
9812
]
},
"GridColumnIndex": 0,
"Label": "Pos23",
"GridRowIndex": 0
}
],
"Interval_ms": 0,
"UUID": "8047334b-bb48-4738-bf85-79f4d0c5822a",
"UserName": "Nikon",
"Depth": 2,
"PixelType": "GRAY16",
"Time": "2019-12-08 14:24:19 -0500",
"Date": "2019-12-08",
"z-step_um": 5,
"MetadataVersion": 10,
"PositionIndex": 14,
"SlicesFirst": true,
"ChContrastMin": [
0,
0,
0,
0,
0
],
"Width": 2048,
"PixelAspect": 1,
"MicroManagerVersion": "1.4.23 20190321",
"ROI": [
0,
0,
2048,
2048
],
"ChNames": [
"Il-G",
"Il-T",
"Il-A",
"Il-C",
"P-DIC"
],
"IJType": 1,
"GridRow": 0,
"Comment": "",
"Height": 2048,
"GridColumn": 0,
"Prefix": "seq_6",
"PixelSize_um": 0.64,
"Frames": 1,
"CameraTimeout": 20000,
"BitDepth": 16,
"KeepShutterOpenChannels": false,
"Source": "Micro-Manager",
"Channels": 5,
"ComputerName": "ZadorConfocal1",
"CustomIntervals_ms": [],
"KeepShutterOpenSlices": true,
"ChColors": [
-13369345,
-256,
-52225,
-1,
-1
],
"TimeFirst": false,
"ChContrastMax": [
65536,
65536,
65536,
65536,
65536
],
"Positions": 24,
"Directory": "F:\\20191206motorcortex117BC1-3"
},
"Source": "Prime BSI",
"PI AO-SupportsTriggering": "0",
"Prime BSI-PVCAM Version": "3.8.0",
"XLIGHT Dichroic-Label": "QUAD",
"COM4-StopBits": "1",
"555 Shutter-TriggerSampleRate": "100000",
"Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW",
"445 AO-MaxVolts": "5.0000",
"405 AO-AutoDetectedOutputPort": "Dev1/ao0",
"TransmittedLamp-Description": "",
"640 Shutter-Name": "AnalogIO",
"FilterTurretOLYMPUS-Name": "FilterCube",
"555 Shutter-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-MaxVolts": "5.0000",
"COM4-DelayBetweenCharsMs": "0.0000",
"LightPath-State": "1",
"520 Shutter-MaxVolts": "5.0000",
"470 Shutter-MaxVolts": "5.0000",
"PI AO-MaxVolts": "5.0000",
"640 AO-IOChannel": "Dev1/ao6",
"555 AO-TriggerSequenceLength": "1000",
"Objective-Description": "Olympus Objective Turret",
"Prime BSI-PP 3 ENABLED": "Yes",
"XLIGHT Emission Wheel-Label": "647/57",
"COM3-Parity": "Even",
"405-State": "0",
"520 Shutter-Volts": "0.0000",
"405-Name": "DA Shutter",
"DA Z Stage-Stage Low Position(um)": "0.0000",
"OlympusHub-Port": "COM3",
"Shutter1-Description": "Shutter Number 1",
"XLIGHT Dichroic-Name": "XLIGHT Dichroic",
"LudlShutter-LudlDeviceNumberShutter": "1",
"LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ",
"PI AO-TriggerSampleRate": "100000",
"Core-Galvo": "",
"Position": "Default",
"CameraChannelIndex": 0,
"SliceIndex": 16,
"Core-Focus": "DA Z Stage",
"445 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-SupportsTriggering": "0",
"FrameIndex": 0,
"COM6-BaudRate": "9600",
"405 AO-Demo": "No",
"TransmittedLamp-State": "1",
"LightPath-ClosedPosition": "",
"Condenser-Label": "Position-2",
"520 AO-TriggerSampleRate": "100000",
"COM4-Parity": "None",
"Core-ImageProcessor": "",
"520 AO-AutoDetectedOutputPort": "Dev1/ao3",
"Prime BSI-Timing-ExposureTimeNs": "100004000.0000",
"XLIGHT Spin motor-Name": "XLIGHT Spin motor",
"Prime BSI-ShutterCloseDelay": "0",
"405 AO-Sequenceable": "1",
"528 Shutter-Volts": "0.0000",
"COM7-Description": "Serial port driver (boost:asio)",
"OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01",
"445 Shutter-Block voltage": "false",
"Core-AutoShutter": "1",
"COM7-DelayBetweenCharsMs": "0.0000",
"445 Shutter-SupportsTriggering": "0",
"PI AO-Description": "NI DAC",
"Prime BSI-Color": "OFF",
"TransmittedLamp-ClosedPosition": "",
"Core-XYStage": "XYStage",
"Prime BSI-PP 3 THRESHOLD": "85",
"Shutter1-State": "0",
"PI AO-MinVolts": "0.0000",
"528 AO-Sequenceable": "1",
"528 Shutter-Name": "AnalogIO",
"405 Shutter-TriggerInputLine": "/Dev1/PFI0",
"640 AO-AutoDetectedOutputPort": "Dev1/ao6",
"405 Shutter-Description": "NI DAC",
"405 Shutter-IOChannel": "Dev1/ao7",
"Core-AutoFocus": "",
"470 Shutter-Volts": "0.0000",
"Objective-State": "0",
"640 Shutter-Description": "NI DAC",
"640 AO-MinVolts": "0.0000",
"BitDepth": 16,
"Slice": 16,
"LudlController-Description": "Ludl Controller (Mac 2000/5000)",
"Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH",
"PI AO-AutoDetectedOutputPort": "Dev1/ao14",
"470 AO-TriggerInputLine": "/Dev1/PFI0",
"COM4-Verbose": "1",
"COM3-Fast USB to Serial": "Enable",
"Prime BSI-ClearMode": "Auto",
"PI AO-Volts": "2.5000",
"Condenser-State": "1",
"555 Shutter-SupportsTriggering": "0",
"Core-Shutter": "LudlShutter",
"528 AO-TriggerSequenceLength": "1000",
"XLIGHT Hub-Port": "COM6",
"Prime BSI-ActualInterval-ms": "22.9500",
"PI AO-IOChannel": "Dev1/ao14",
"520 AO-MaxVolts": "5.0000",
"520 Shutter-Description": "NI DAC",
"ElapsedTime-ms": 362318,
"640 Shutter-Sequenceable": "1",
"PI AO-Name": "AnalogIO",
"470 Shutter-Description": "NI DAC",
"445-State": "0",
"Frame": 0,
"520-Name": "DA Shutter",
"ManualFocus-Description": "Manual Focus (Z-Drive)",
"Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW",
"Prime BSI-AcquisitionMethod": "Callbacks",
"PositionName": "Pos14",
"XLIGHT Spin motor-Label": "On (spinning)",
"640 AO-TriggerSampleRate": "100000",
"520 Shutter-SupportsTriggering": "0",
"Prime BSI-CircularBufferEnabled": "ON",
"Height": 2048,
"555 AO-TriggerInputLine": "/Dev1/PFI0",
"470 Shutter-TriggerSampleRate": "100000",
"Objective-Use Safe Position": "1",
"555 AO-Demo": "No",
"LudlController-TransmissionDelay": "0",
"COM6-Handshaking": "Off",
"Prime BSI-PP 5 LAMBDA": "26",
"FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes",
"445 Shutter-Name": "AnalogIO",
"COM3-Name": "COM3",
"445 Shutter-TriggerSampleRate": "100000",
"LudlController-Reset": "Operate",
"520 Shutter-Name": "AnalogIO",
"470 AO-Name": "AnalogIO",
"XLIGHT Hub-1-Description": "XLIGHT hub",
"640 Shutter-MinVolts": "0.0000",
"Prime BSI-FullWellCapacity": "45000",
"COM3-DelayBetweenCharsMs": "0.0000",
"PixelType": "GRAY16",
"Prime BSI-PP 2 ENABLED": "Yes",
"Prime BSI-PP 7 Reset": "No",
"640 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-ChipName": "GS2020",
"Prime BSI-Trigger-Expose Out-Mux": "1",
"COM7-Name": "COM7",
"528 AO-TriggerInputLine": "/Dev1/PFI0",
"640 Shutter-SupportsTriggering": "0",
"COM7-DataBits": "8",
"Condenser-Description": "Transmitted Light Condenser",
"520 AO-Demo": "No",
"470 AO-AutoDetectedOutputPort": "Dev1/ao2",
"445 AO-IOChannel": "Dev1/ao1",
"Prime BSI-Timing-PreTriggerDelayNs": "0.0000",
"Prime BSI-PP 1 ENABLED": "Yes",
"Prime BSI-TransposeMirrorX": "0",
"ManualFocus-FarLimit": "0.0100",
"Prime BSI-TransposeMirrorY": "0",
"XLIGHT Disk slider-Name": "XLIGHT Disk slider",
"COM6-Name": "COM6",
"405 Shutter-Sequenceable": "1",
"445 Shutter-MinVolts": "0.0000",
"FilterTurretOLYMPUS-ClosedPosition": "",
"528 AO-Demo": "No",
"COM6-Fast USB to Serial": "Disable",
"445 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-TransposeCorrection": "0",
"405 Shutter-AutoDetectedOutputPort": "Dev1/ao7",
"528 Shutter-TriggerSampleRate": "100000",
"COM7-AnswerTimeout": "500.0000",
"405 AO-Volts": "0.0000",
"555 AO-IOChannel": "Dev1/ao5",
"COM7-Handshaking": "Off",
"528-State": "0",
"640-Name": "DA Shutter",
"640 Shutter-AutoDetectedOutputPort": "Dev1/ao13",
"445 Shutter-AutoDetectedOutputPort": "Dev1/ao8",
"Prime BSI-Gain": "1-HDR",
"FilterTurretOLYMPUS-Label": "Position-6",
"405 Shutter-TriggerSequenceLength": "1000",
"640 Shutter-Block voltage": "false",
"528 Shutter-IOChannel": "Dev1/ao11",
"Prime BSI-ClearCycles": "2",
"555 Shutter-Demo": "No",
"XYStage-StepSizeX_um": "1.0000",
"640 AO-Name": "AnalogIO",
"Objective-Safe Position": "100.0000",
"PI AO-Sequenceable": "1",
"470 AO-Sequenceable": "1",
"OlympusHub-Model": "IX2",
"Prime BSI-Exposure": "10.00",
"Prime BSI-ExposeOutMode": "Rolling Shutter",
"520 Shutter-MinVolts": "0.0000",
"XLIGHT Hub-1-Port": "COM6",
"COM6-Parity": "None",
"470 Shutter-TriggerInputLine": "/Dev1/PFI0",
"COM7-Parity": "None",
"640 AO-TriggerSequenceLength": "1000",
"520 AO-Sequenceable": "1",
"Prime BSI-Description": "PVCAM API device adapter",
"405 Shutter-Block voltage": "false",
"520 Shutter-TriggerInputLine": "/Dev1/PFI0",
"555 AO-TriggerSampleRate": "100000",
"Prime BSI-CircularBufferAutoSize": "ON",
"Objective-Label": "10x",
"555 Shutter-Sequenceable": "1",
"640 Shutter-Volts": "0.0000",
"LudlController-Name": "LudlController",
"ManualFocus-NearLimit": "30000.0000",
"555 Shutter-Name": "AnalogIO",
"XLIGHT Spin motor-State": "1",
"445 AO-Demo": "No",
"470 AO-IOChannel": "Dev1/ao2",
"Prime BSI-ShutterOpenDelay": "0",
"COM7-StopBits": "2",
"555-State": "0",
"Prime BSI-Offset": "100",
"COM3-AnswerTimeout": "500.0000",
"520 AO-Name": "AnalogIO",
"XLIGHT Dichroic-Description": "XLIGHT Dichroic filter",
"555 AO-SupportsTriggering": "0",
"COM7-Fast USB to Serial": "Disable",
"Prime BSI-PP 2 MIN ADU AFFECTED": "200",
"XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel",
"520 Shutter-Block voltage": "false",
"Prime BSI-Timing-PostTriggerDelayNs": "11200.0000",
"640-DA Device": "640 Shutter",
"640 AO-SupportsTriggering": "0",
"470-Description": "DA device that is used as a shutter",
"XLIGHT Emission Wheel-State": "2",
"528 AO-TriggerSampleRate": "100000",
"DA Z Stage-Stage High Voltage": "5.0000",
"405 Shutter-Demo": "No",
"LightPath-Description": "Light Path Prism",
"528 AO-AutoDetectedOutputPort": "Dev1/ao4",
"OlympusHub-Description": "Olympus Hub",
"520 AO-MinVolts": "0.0000",
"Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH",
"PixelSizeUm": 0.64,
"528 AO-MaxVolts": "5.0000",
"445 Shutter-MaxVolts": "5.0000",
"640 AO-Block voltage": "false",
"555 AO-Name": "AnalogIO",
"528 Shutter-TriggerSequenceLength": "1000",
"COM4-DataBits": "8",
"520 Shutter-Sequenceable": "1",
"405 Shutter-Volts": "0.0000",
"COM3-DataBits": "8",
"DA Z Stage-Stage High Position(um)": "220.0000",
"470 AO-Demo": "No",
"555 AO-Sequenceable": "1",
"Condenser-ClosedPosition": "",
"Prime BSI-ReadoutRate": "100MHz 16bit",
"Binning": "1x1",
"405 Shutter-SupportsTriggering": "0",
"555 AO-Block voltage": "false",
"528 AO-SupportsTriggering": "0",
"520 Shutter-TriggerSampleRate": "100000",
"470 Shutter-Name": "AnalogIO",
"Shutter1-Name": "Shutter1",
"COM4-Description": "Serial port driver (boost:asio)",
"COM3-Description": "Serial port driver (boost:asio)",
"520 Shutter-IOChannel": "Dev1/ao10",
"Prime BSI-PP 4 ENABLED": "Yes",
"Prime BSI-PreampDelay": "0",
"405 AO-Block voltage": "false",
"405 Shutter-Name": "AnalogIO",
"XYStage-TransposeMirrorY": "0",
"445 AO-AutoDetectedOutputPort": "Dev1/ao1",
"XPositionUm": 414,
"XYStage-TransposeMirrorX": "0",
"COM6-DataBits": "8",
"OlympusHub-Name": "OlympusHub",
"470-DA Device": "470 Shutter",
"640 AO-TriggerInputLine": "/Dev1/PFI0",
"528 Shutter-Block voltage": "false",
"FileName": "img_000000000_P-DIC_016.tif",
"528 Shutter-Description": "NI DAC",
"XYStage-Acceleration": "100",
"555 Shutter-Description": "NI DAC",
"LightPath-Name": "LightPath",
"445 AO-Description": "NI DAC",
"470 AO-Volts": "0.0000",
"ZPositionUm": 150,
"528-DA Device": "528 Shutter",
"640 AO-Description": "NI DAC",
"Channel": "P-DIC",
"XLIGHT Excitation Wheel-Label": "Quad",
"470 Shutter-Sequenceable": "1",
"528 Shutter-Demo": "No",
"405 Shutter-MinVolts": "0.0000",
"Prime BSI-CircularBufferFrameCount": "16",
"528 Shutter-AutoDetectedOutputPort": "Dev1/ao11",
"Prime BSI-PP 5 GAIN": "100",
"LightPath-Label": "Side Port",
"XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position",
"640 Shutter-MaxVolts": "5.0000",
"COM6-Description": "Serial port driver (boost:asio)",
"555 AO-MinVolts": "0.0000",
"640 Shutter-Demo": "No",
"DA Z Stage-DA Device": "PI AO",
"555 Shutter-TriggerSequenceLength": "1000",
"Camera": "",
"ManualFocus-FocusSensitivity": "100",
"Prime BSI-PMode": "Normal",
"445 AO-Sequenceable": "1",
"Prime BSI-TransposeXY": "0",
"Prime BSI-PP 4 MIN ADU AFFECTED": "200",
"520 AO-SupportsTriggering": "0",
"XLIGHT Hub-Name": "XLIGHT Hub",
"445 Shutter-TriggerInputLine": "/Dev1/PFI0",
"XYStage-StepSizeY_um": "1.0000",
"PI AO-Block voltage": "false",
"520 AO-IOChannel": "Dev1/ao3",
"Prime BSI-PP 6 ENABLED": "No",
"470 Shutter-Demo": "No",
"555 AO-MaxVolts": "5.0000",
"520-DA Device": "520 Shutter",
"LudlShutter-Name": "LudlShutter",
"405 AO-Name": "AnalogIO",
"405 AO-TriggerSampleRate": "100000",
"XLIGHT Dichroic-State": "1",
"NextFrame": 0,
"ManualFocus-Position": "8600.6500",
"528-Description": "DA device that is used as a shutter",
"TransmittedLamp-Label": "On",
"445 Shutter-Sequenceable": "1",
"555 AO-AutoDetectedOutputPort": "Dev1/ao5",
"COM6-StopBits": "1",
"PI AO-TriggerInputLine": "/Dev1/PFI0",
"Time": "2019-12-08 14:24:41 -0500",
"405 AO-IOChannel": "Dev1/ao0",
"COM7-Verbose": "1",
"COM4-Name": "COM4",
"COM4-BaudRate": "9600",
"445 Shutter-Description": "NI DAC",
"640-State": "0",
"470 AO-Block voltage": "false",
"528 AO-Volts": "0.0000",
"640 AO-MaxVolts": "5.0000",
"Prime BSI-MetadataEnabled": "No",
"445 AO-Block voltage": "false",
"520-Description": "DA device that is used as a shutter",
"Prime BSI-PreampOffLimit": "10000",
"COM6-Verbose": "0",
"555 Shutter-Block voltage": "false",
"445 AO-TriggerSequenceLength": "1000",
"520 AO-Block voltage": "false",
"Prime BSI-TriggerMode": "Internal Trigger",
"405 AO-TriggerInputLine": "/Dev1/PFI0",
"ChannelIndex": 4,
"Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000",
"445 AO-MinVolts": "0.0000",
"COM7-BaudRate": "9600",
"Core-TimeoutMs": "5000",
"470 Shutter-TriggerSequenceLength": "1000",
"Prime BSI-Timing-ClearingTimeNs": "0.0000",
"640 Shutter-TriggerInputLine": "/Dev1/PFI0",
"Prime BSI-SerialNumber": "A18J204029",
"555-Description": "DA device that is used as a shutter",
"Prime BSI-Timing-ReadoutTimeNs": "22937000.0000",
"Prime BSI-Binning": "1x1",
"Prime BSI-PP 1 THRESHOLD": "115",
"Width": 2048,
"445 AO-SupportsTriggering": "0",
"TransmittedLamp-Name": "",
"DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board",
"470 AO-SupportsTriggering": "0",
"COM3-StopBits": "2",
"528 Shutter-MinVolts": "0.0000",
"COM3-Verbose": "1",
"Prime BSI-PP 5 ENABLED": "No",
"640 AO-Volts": "0.0000",
"FilterTurretOLYMPUS-State": "5",
"528-Name": "DA Shutter",
"470 Shutter-IOChannel": "Dev1/ao9",
"OlympusHub-Control": "Manual + Computer",
"520 Shutter-Demo": "No",
"XLIGHT Hub-Description": "XLIGHT hub",
"555 Shutter-IOChannel": "Dev1/ao12",
"XYStage-Description": "Prior XY stage driver adapter",
"Prime BSI-PixelType": "16bit",
"Prime BSI-Y-dimension": "2048",
"Prime BSI-Trigger Timeout (secs)": "10",
"555 AO-Description": "NI DAC",
"COM4-Handshaking": "Off",
"XLIGHT Excitation Wheel-State": "1",
"405 AO-SupportsTriggering": "1",
"XYStage-SCurve": "100",
"528 AO-Name": "AnalogIO",
"405 AO-TriggerSequenceLength": "1000",
"LudlController-Port": "COM7",
"XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position",
"555 AO-Volts": "0.0000",
"405 Shutter-TriggerSampleRate": "100000",
"COM4-AnswerTimeout": "500.0000",
"YPositionUm": 28632,
"445 AO-Name": "AnalogIO",
"ManualFocus-FocusWheel": "Off",
"555 Shutter-Volts": "0.0000",
"XLIGHT Hub-1-Name": "XLIGHT Hub",
"COM6-AnswerTimeout": "5000.0000",
"528 AO-IOChannel": "Dev1/ao4",
"LudlShutter-State": "0",
"DA Z Stage-Position": "110.0000",
"445 AO-Volts": "0.0000",
"XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel",
"470 AO-TriggerSampleRate": "100000",
"Prime BSI-PP 5 OFFSET": "25",
"TransmittedLamp-Voltage": "4.7000",
"COM3-BaudRate": "19200",
"Prime BSI-FirmwareVersion": "23.22",
"COM4-Fast USB to Serial": "Disable",
"470 Shutter-AutoDetectedOutputPort": "Dev1/ao9"
}
}