scanner3d { scanner2d { frequency_hz: 7920 nscans: 768 line_duty_cycle: 0.99 daq { kind: NIDAQ nidaq { ao_samples_per_waveform: 16448 trigger: "APFI1" ctr: "ctr0" armstart: "PFI15" name: "Dev1" level_volts: 0.5 flyback_scans: 1 frame_trigger_out: "PFI1" } } digitizer { kind: Alazar alazar { system_id: 1 sample_rate: SAMPLE_RATE_125MSPS channels { board_id: 0 chan_id: CHANNEL_A range: INPUT_RANGE_PM_1_V enabled: true } channels { board_id: 0 chan_id: CHANNEL_B range: INPUT_RANGE_PM_1_V enabled: true } aux_out_board_id: 1 } } pockels { kind: NIDAQ laser: Chameleon name: "Chameleon" calibration { calibrated: true v_zero: 0 v_max: 0.85 } nidaq { v_lim_max: 2 v_lim_min: -2 v_open: 0.56666666666666676 v_closed: 0 ao_channel: "ao2" } simulated { } } pockels { kind: NIDAQ laser: Fianium name: "Fidelity (1064nm)" calibration { calibrated: true v_zero: 0 v_max: 1.4 } nidaq { v_lim_max: 2 v_lim_min: -2 v_open: 0.51661416739176347 v_closed: 0 ao_channel: "ao3" } simulated { } } shutter { kind: Simulated simulated { } } linear_scan_mirror { kind: NIDAQ nidaq { v_lim_max: 10 v_lim_min: -10 vpp: 13.2 ao_channel: "ao0" } } } zpiezo { kind: NIDAQ nidaq { um2v: 0.03982 v_lim_max: 10 v_lim_min: -3 v_offset: 0 channel: "ao1" } um_max: 250 um_min: 0 um_step: 1 } zref_um: 0 } stage { kind: C843 c843 { id: 1 axis { id: 1 stage: "M-511.DD_NEW_X" } axis { id: 2 stage: "M-511.DD_NEW_Y" } axis { id: 3 stage: "M-405.CG_NEW_Z" } } default_velocity_mm_per_sec { x: 2 y: 4 z: 0.3 } last_target_mm { x: 72.0886 y: 14.23455 z: 32.911957 } tilemode: PixelAligned use_two_dimensional_tiling: true tile_z_offset_mm: -0.014642862603068352 } vibratome { kind: Serial serial { port: "COM2" amplitude: 255 } feed_axis: Y feed_vel_mm_per_sec: 0.1 feed_mm: 23 geometry { cut_pos_mm { x: 67.024002075195313 y: 70.991996765136719 } dz_mm: 1.8150005340576172 } cut_thickness_um: 175 } fov { x_size_um: 384.72339 y_size_um: 456.35 z_size_um: 250 x_overlap_um: 60 y_overlap_um: 25 z_overlap_um: 75 rotation_radians: 0 } pipeline { frame_average_count: 1 downsample_count: 4 } file_series { root: "G:\\Data" date: "2018-10-04" seriesno: 2731 pathsep: "\\" } file_prefix: "ngc" stack_extension: ".tif" config_extension: ".microscope" autotile { z_um: 175 maxz_mm: 42.5 timeout_ms: 15000 ichan: 0 intensity_threshold: 11500 area_threshold: 0.1 search_radius: 2 use_adaptive_tiling: true use_explore: true } surface_probe { simulated { } } surface_scan { } time_series { interval_ms: 300000 } surface_find { backup_frac: 0.8 max_backup_um: 125 max_raise_um: 40 min_um: 0 max_um: 150 dz_um: 5 ichan: 0 intensity_threshold: 11800 area_threshold: 0.1 offset_um: 0 } adaptive_tiling { every: 3 mindist: 2 } trip_detect { threshold { ichan: 0 intensity_threshold: 11400 area_threshold: 0.0001 } threshold { ichan: 1 intensity_threshold: 16100 area_threshold: 0.0001 } frame_threshold: 1500 } pmt { simulated { } } backup_distance_mm: 2.5