1.0 0 true false 16 2 1 3 1 1920 0 0 1920 0 10 512 512 1324 Average Frame 1 false false 0 1 1 1 EC Plan-Neofluar 5x/0.16 0 0 0.0052177820000000007 0.0016167550000000001 1.5999999999999999e-006 0 OnTheFlyByTrigger 1 512 512 3.0000000000000001e-005 0 0 512 512 1 0.69999999999999996 1.304745172134391e-006 1.304745172134391e-006 6.1073949999999998e-006 3 false ZStack false 1 1 false false false false Cubic 5.6100000000000001e-007 0.14999999999999999 false 0 Laser 561-20 0 0 0 0 MainBeamSplitterNonDescanned LBF 405/488/561/640 0 CameraBeamSplitter SBS LP 560 3 BeamSplitterInvalid -1 CameraMonochrome2 false TV2 0 0 0.10000000000000001 0 32 LsmDetectorPalette_0_0 #00FF00 Integration 1 0 true BP 575-615 Off 1 1 1.1168976306040343e-308 6.9230114703934895e-313 8.665327634996502e-304 3.8196013548633569e-313 0 -1 -1 -1 -1 0.00998670376 SPIM 0 0 None 0 true 0 6.3800000000000007e-007 0.14000000000000001 false 0 Laser 638-75 0 0 0 0 MainBeamSplitterNonDescanned LBF 405/488/561/640 0 CameraBeamSplitter SBS LP 640 4 BeamSplitterInvalid -1 CameraMonochrome2 false TV2 0 0 0.10000000000000001 0 32 LsmDetectorPalette_1_0 #FF0000 Integration 1 0 true LP 660 Off 1 1 -2.8152046383240938e-309 0 6.9518567794014654e-310 1.2070498967037721e-311 0 -1 -1 -1 -1 0.00998670376 SPIM 0 0 None 0 true 0 4.8800000000000003e-007 0.26899850000000003 false 0 Laser 488-50 0 0 0 0 MainBeamSplitterNonDescanned LBF 405/488/561/640 0 CameraBeamSplitter SBS LP 490 1 BeamSplitterInvalid -1 CameraMonochrome2 false TV2 0 0 0.10000000000000001 0 32 LsmDetectorPalette_2_0 #FFFFFF Integration 1 0 true BP 505-545 Off 1 1 1.2071282853962928e-311 1.2071282861551776e-311 1.2071282861077473e-311 1.2071282867954867e-311 0 -1 -1 -1 -1 0.00749002782 SPIM 0 0 None 0 true 0 Off 0 ConvexHull 5 5 0.050000000000000003 0.050000000000000003 Laser 488-50 0.02 Laser 561-20 0.059999999999999998 Laser 638-75 <LsmTag Name="CarlZeissAim.LightSheetOffsetZRight.638" Type="double">-0.24800000000000008</LsmTag> C(2,3) <AuditTrail ProcessingSteps="1"> <CreateSubsetKeepStructure Timestamp="2025-02-04T18:56:36.0998595-05:00" ElapsedTime="00:47:46.9443654" ID="1"> <OverruleMultiChannelParameterIndex>-1</OverruleMultiChannelParameterIndex> <MultiChannelParameterIndex>-1</MultiChannelParameterIndex> <CanProcessInplace>false</CanProcessInplace> <SubsetString>C(2,3)</SubsetString> <KeepMosaicTiles>false</KeepMosaicTiles> <PropagateRoi>false</PropagateRoi> <ChannelProcessingMode> <c0> <Name>Cam2-T1</Name> <Index>0</Index> <ChannelProcessingMode>ProcessChannel</ChannelProcessingMode> </c0> <c1> <Name>Cam2-T2</Name> <Index>1</Index> <ChannelProcessingMode>ProcessChannel</ChannelProcessingMode> </c1> <c2> <Name>Cam2-T3</Name> <Index>2</Index> <ChannelProcessingMode>ProcessChannel</ChannelProcessingMode> </c2> </ChannelProcessingMode> <Input>Z:\MicroscopyCore\GuestAccess\Melissa\TID MOM CTB oct 2022\Female 4 488register 555streptavidin 647CTB.czi</Input> </CreateSubsetKeepStructure> </AuditTrail> False 0.74209620436634 zeiss ZEN (blue edition) 3.4.91.00000 Female 4 488register 555streptavidin 647CTB Light sheet thickness: 13.37 µm Illumination mode: dual (mean fused) Pivot scan: on Z-Stack mode: Continuous Drive Tiles overlap: 5.0 % Track switch mode: Z-Stack Laser blocking filter: FW1 : LBF 405/488/561/640 Emission selection: 555 CAM BS : SBS LP 560 555 EF1 : BP 575-615 647 CAM BS : SBS LP 640 647 EF1 : LP 660 488 CAM BS : SBS LP 490 488 EF1 : BP 505-545 zeiss 2022-11-01T15:18:07 Image 0 true Bgr24 8 7391 11039 1324 2 24 1 1 1 1 1 1 1 Uncompressed 100 Oblique Fluorescence 648.00000000000011 9986703.7599999998 Unspecified Bgr24 8 SPIM 638.00000000000011 648.00000000000011 1x1 0 0 0 0.10000000000000001 0.85999999999999999 638.00000000000011 0 0 0 0 0.014674698900146461 6 0.15367587226518806 Off 1 1 -2.8152046383240938E-309 0 6.95185677940147E-310 1.2070498967037721E-311 Oblique Fluorescence 498.00000000000006 7490027.8200000003 Unspecified Bgr24 8 SPIM 488.00000000000006 498.00000000000006 1x1 0 0 0 0.10000000000000001 0.73100149999999997 488.00000000000006 0 0 0 0 0.014674698900146461 6 0.15367587226518806 Off 1 1 1.2071282853962928E-311 1.20712828615518E-311 1.20712828610775E-311 1.20712828679549E-311 2022-11-01T15:18:07-04:00 TimeStamps 0 0 6.1073949999999995 Meander Rectangle true 3 true 3 0 270 180 Air 1 0.69999999999999996 0 0 Lightsheet Z.1 50 Laser 488-50 488.000000 20 Laser 561-20 561.000000 60 Laser 638-75 638.000000 1 0.69999999999999996 0 Camera pco.edge 5.5 1 0.69999999999999996 0 Camera pco.edge 5.5 1 0.69999999999999996 0 Camera pco.edge 5.5 Air 0.16000000000000003 5 EC Plan-Neofluar 5x/0.16 (Air : n=1.000) Measured 02/11/2025 22:17:53 1.304745172134391E-06 1.304745172134391E-06 6.107395E-06 8 Bgr24 Dye2 648.00000000000011 #FFFF0000 #FFFF0000 8 Bgr24 Dye3 498.00000000000006 #FFFFFFFF #FFFFFFFF None 3 15 0